To test this model with a biologically relevant, visible chromatin locus, we transiently introduced a GFP ER deletion series, including 1 GFP ER 282 AF1 with the DNA binding domain, DBD, 2 GFP ER 534 AF1, DBD and LBD up to, but excluding, helix 12 and, 3 GFP ER 554 AF1, DBD and LBD including helix 12, Fig <a href=https://acialis.one>cialis for sale online</a>